Divinyl sulfone as a cross-linking reagent for oligomeric proteins |
| |
Authors: | Sereĭkaĭte I Bassus D Bobnis R Denis G Bumialene Zh Bumialis B A |
| |
Institution: | Faculty of Fundamental Sciences, Gediminas Technical University, Vilnius, Sauletekio al. 11, Vilnius, 2040 Lithuania. sjolanta@fm.vtu.lt |
| |
Abstract: | The kinetics of the nucleophilic addition reactions of divinyl sulfone to amino groups of glycine and model proteins was studied in aqueous solution at 30 degrees C. The rate constants for glycine, bovine serum albumin, and alpha 1-casein were (4.84 +/- 0.58) x 10(-1), (2.97 +/- 0.31) x 10(-2), and (2.38 +/- 0.49) x 10(-2) M-1s-1, respectively. Divinyl sulfone was proposed as a cross-linking reagent for the qualitative detection of protein association in solution. The cross-linking capacity of divinyl sulfone was compared to that of 1,3,5-triacryloylhexahydro-s-triazine. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|