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Isolation and characterization of ten microsatellite loci for Senegal sole (Solea senegalensis Kaup)
Authors:VICTORIA FUNES  EUGENIA ZUASTI  GAETANO CATANESE  CARLOS INFANTE  MANUEL MANCHADO
Abstract:Senegal sole (Solea senegalensis Kaup) is a high‐value marine flatfish exploited in both fisheries and aquaculture. Here we describe the isolation of seven tetranucleotide and three dinucleotide polymorphic microsatellite loci. Characteristics of a captive broodstock and a wild population are described. Three loci displayed a significant departure from Hardy–Weinberg expectations in both populations, suggesting a substantial frequency of null alleles. The remaining seven loci were found to be in equilibrium in the wild population, whereas only four of them were in the cultured population. Cross‐species amplification was successful for three loci in other five flatfishes.
Keywords:cross‐amplification  microsatellites  polymorphism  Senegal sole
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