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Microsatellite DNA markers for parental assignment in hybrid striped bass (Morone saxatilis × Morone chrysops)
Authors:Kirstin Ross  Xiaoxue Wang  Kathleen G O'Malley  Delbert M Gatlin  John R Gold
Abstract:Development of nine polymorphic microsatellites from a genomic library of hybrid striped bass (female Morone chrysops × male Morone saxatilus) DNA is described. Breeding of hybrid striped bass for aquaculture is based largely on breeding wild fish. Molecular markers such as microsatellites will be useful tools for developing broodstock, estimating heritability for production traits, and selective breeding via marker‐assisted selection. The nine polymorphic microsatellites include six dinucleotide and three complex repeat motifs. The number of alleles detected among a sample of 10 individuals of each species was relatively low. All polymerase chain reaction primer pairs also amplified products in the sea bass Dicentrarchus labrax.
Keywords:genome library  hybrid striped bass  microsatellites  polymerase chain reaction primers
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