Characterization of microsatellite loci in Ulmus minor Miller and cross‐amplification in U. glabra Hudson and U. laevis Pall. |
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| Authors: | C COLLADA P FUENTES-UTRILLA L GIL M T CERVERA |
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| Institution: | 1. Departamento de Biotecnología. ETSIM, Ciudad Universitaria s/n, 28040 Madrid, Spain;2. Unidad de Anatomía, Fisiología y Genética Vegetal. ETSIM, Ciudad Universitaria s/n, 28040 Madrid, Spain |
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| Abstract: | We developed five microsatellites from Ulmus minor using an AFLP protocol followed by an enrichment step, with biotinylated primers containing tandem repeats. Loci were characterized using a total of 30 elms from six Spanish populations. Three microsatellites were also transferred to U. laevis and U. glabra. |
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| Keywords: | cross‐amplification microsatellite U glabra U laevis Ulmus minor |
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