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Isolation and characterization of polymorphic microsatellite loci in Acanthoscelides obtectus Say (Coleoptera: Bruchidae)
Authors:N. ALVAREZ  C. BORN  A.‐M. RISTERUCCI  P. SOURROUILLE  B. BENREY  M. HOSSAERT‐MCKEY
Abstract:Six microsatellite loci were isolated from the bruchid Acanthoscelides obtectus Say (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from 3 to 18. We found high levels of within‐population variation at most loci, with heterozygosities ranging from 0 to 0.75. Cross‐species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obvelatus Bridwell and A. argillaceus Sharp.
Keywords:    Acanthoscelides obtectus     Bruchidae  Coleoptera  microsatellite  pest species      Phaseolus   
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