In Vitro Morphogenesis in Nardostachys jatamansi DC.: Shoot Regeneration from Callus Derived Roots |
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| Authors: | MATHUR JAIDEEP |
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| Institution: | Plant Tissue Culture Laboratory, Department of Botany, University of Gorakhpur Gorakhpur-273 009, India |
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| Abstract: | Callus cultures of Nardostachys jatamansi DC. maintained onMurashige and Skoog's medium containing 3.0 mg 1–1 of -naphthaleneacetic acid and 0.25 mg 1–1 of kinetin whenshifted to medium containing 0.25–1.0 mg 1–1 ofindole-3-acetic acid or indole-3-butync acid showed profuserhizogenesis. The callus-regenerated roots when transferredto medium containing 2.0–6.0 mg 1–1 of kinetin producedshoot buds. The de novo shoot bud regeneration took place eitherdirectly from cortical cells or from the inner stelar region.In addition, direct, concomitant root-shoot development wasalso observed. Nardostachys jatamansi, organogenesis, root-buds |
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