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Action spectrum for light-induced formation of adventitious shoots in hairy roots of horseradish
Authors:Tsutomu Saitou  Yoshinobu Tachikawa  Hiroshi Kamada  Masakatsu Watanabe  Hiroshi Harada
Affiliation:(1) Gene Experiment Center, Institute of Biological Sciences, University of Tsukuba, 305 Tsukuba-shi, Ibaraki, Japan;(2) National Institute for Basic Biology, Okazaki National Research Institutes, Large Spectrograph Laboratory, Myodaiji-cho, Okazaki, 444 Aichi, Japan
Abstract:An action spectrum was determined for lightinduced formation of adventitious shoots in hairy roots of horseradish (Armoracia rusticana Gaert., Mey. et Scherb.) cultured in vitro. Near ultraviolet (350–400 nm), blue (440–460 nm) and red light (600–680 nm) were most effective in inducing formation of adventitious buds. Farred light (730 nm) inhibited the promotive effect of all three wavelength regions. These results are consistent with induction by phytochrome(s) of adventitious shoots in hairy roots of horseradish.
Keywords:Action spectrum  Adventitious bud formation  Armoracia  Hairy roots  Light and bud formation  Phytochrome
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