Incubation of corn coleoptiles with auxin enhances in-vitro fusicoccin binding

Binding of fusicoccin (FC) to microsomal preparations of corn (Zea mays L.) coleoptiles is enhanced after incubation of the tissue with indole-3-acetic acid (IAA). Treatment of the kinetic data according to Scatchard shows that the enhancement is a consequence of an increase in the number of high-affinity FC-binding sites without changes of their K_D. The minimal effective concentration of IAA is 10^-7 M; above 10^-5 M the effect declines. The stimulation is insensitive to protein-synthesis inhibitors (cycloheximide and puromycin). The same effect is observed with the synthetic auxins 2,4-dichlorophenoxyacetic acid and naphtalene-1-acetic acid while it is abolished by the auxin antagonists naphtalene-2-acetic acid and p-chlorophenoxyisobutyric acid. Since the above effect is only observed with intact tissue and not after incubation of IAA with microsomal preparations, a direct interaction of IAA with the FC-binding sites is ruled out and an alternative mechanism must be sought.Abbreviations 2,4-D 2,4-dichlorophenoxyacetic acid - FC fusicoccin - ³H]FC ³H-labeled dihydrofusicoccin - IAA indole-3-acetic acid - 1-NAA naphtalene-1-acetic acid - 2-NAA naphtalene-2-acetic acid - PCIB p-chlorophenoxyisobutyric acid