Physical mapping of genes in somatic cell radiation hybrids by comparative genomic hybridization to cDNA microarrays |
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| Authors: | Lin Johann Y Pollack Jonathan R Chou Fan-Li Rees Christian A Christian Allen T Bedford Joel S Brown Patrick O Ginsberg Mark H |
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| Affiliation: | (1) Department of Vascular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA;(2) Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA;(3) Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA;(4) Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA;(5) Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA;(6) Department of Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA; |
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| Abstract: | Background Somatic cell mutants can be informative in the analysis of a wide variety of cellular processes. The use of map-based positional cloning strategies in somatic cell hybrids to analyze genes responsible for recessive mutant phenotypes is often tedious, however, and remains a major obstacle in somatic cell genetics. To fulfill the need for more efficient gene mapping in somatic cell mutants, we have developed a new DNA microarray comparative genomic hybridization (array-CGH) method that can rapidly and efficiently map the physical location of genes complementing somatic cell mutants to a small candidate genomic region. Here we report experiments that establish the validity and efficacy of the methodology. |
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