Media optimization for maximum biomass production in cell cultures of pacific yew |
| |
Authors: | R E B Ketchum D M Gibson L Greenspan Gallo |
| |
Institution: | (1) Plant Protection Research Unit. USDA, ARS, NAA, U.S. Plant, Soil, and Nutrition Laboratory, Cornell University, Tower Road, 14853 Ithaca, NY, USA |
| |
Abstract: | Three cell lines of Taxus brevifolia Nutt. with differing growth rates were used to assess the effects of basal salt mixtures, carbohydrates, organic nitrogen additives, vitamin formulations, and plant growth regulators on callus growth. Gamborg's B5 major salts provided significantly better growth than all other salt formulations tested. The greatest biomass was obtained with 1% total carbohydrate. The best carbohydrate combination, 0.5% fructose + 0.5% sucrose, was significantly better than all other combinations of carbohydrates tested. A complex vitamin mixture was significantly better than any one previously published vitamin formulation. Greatest rates of callus growth were obtained with 4.14 M (1 mg l-1 picloram, 0.46 M (0.1 mg l-1 kinetin, and 0.38 M (0.1 mg l-1) abscisic acid or 0.29 M (0.1 mg l-1 gibberellic acid. Our final medium, TM5, is superior to published methods for the general callus culture of T. brevifolia. This medium has improved growth in three tested cell lines to provide doubling times of 3.5 to 5.6 days, an average 5.3-fold increase over our previously published medium.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- 2,4,5-T
2,4,5-trichlorophenoxyacetic acid, 2ip-6-( , -dimethylamino)-purine
- ABA
abscisic acid
- BA
6-benzyladenine
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- kinetin
6-furfurylaminopurine
- NAA
napthaleneacetic acid
- picloram
4-amino-3,5,6-trichloropicolinic acid |
| |
Keywords: | taxol tissue culture Taxus brevifolia |
本文献已被 SpringerLink 等数据库收录! |
|