Abstract: | 1. The binding of glycocholic acid, chenodeoxycholic acid and lithocholic acid to rat liver 1000 000g supernatants was studied by equilibrium dialysis. 2. The binding characteristics of the bile acids suggest that the binding components are involved in bile acid transport. 3. When mixtures of 14C]lithocholic acid and liver supernatants were eluted from columns of Sephadex G-75, a prominent peak of 14C]lithocholic acid appeared with proteins of mol.wt. approx. 40000. A second, smaller, peak of 14C]lithocholic acid was eluted with proteins of mol.wt. approx. 100000. 4. The inclusion of cholic acid, glycocholic acid or chenodeoxycholic acid in the eluting buffer decreased the amount of 14C]lithocholic acid that was eluted with the higher-molecular-weight component. |