Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4 |
| |
Authors: | Komoto Satoshi Kugita Masanori Sasaki Jun Taniguchi Koki |
| |
Institution: | Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan. |
| |
Abstract: | Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the corresponding sequence of a different P-type DS-1 (human RV strain, G2P4]). Serological analyses with a panel of anti-VP4- and -VP7-neutralizing monoclonal antibodies revealed that the rescued virus carries a novel antigenic mosaic of cross-reactive neutralization epitopes on its VP4 surface. This is the first report of the generation of a recombinant RV with artificial amino acid substitutions. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|