Immunological properties of a radioiodinated monoclonal antibody and its F(ab')2mu fragments directed against the polymorphic epithelial mucin of human breast cancer. |
| |
Authors: | M Liberatore M Nuti G Cascialli V Turchi M Clemente G Rossetto A Centi Colella A Pala |
| |
Institution: | Section of Nuclear Medicine, University of Roma, La Sapienza, Italy. |
| |
Abstract: | The purification of the IgM monoclonal antibody 436 against a breast tumor antigen from mouse ascitic fluid is reported. The purified immunoglobulin was radioiodinated and the resulting product assessed for its binding capacity and binding specificity. Purified IgM-436 served for F(ab')2 mu preparation which was tested for its antigen binding capacity. Radioiodinated IgM-436 and its F(ab')2 mu retained their immunological activity which was never lower than those of the corresponding cold products. |
| |
Keywords: | |
|