人受精促进肽受体基因（TCP11b）的剪接、克隆和差别表达

运用同源比较和PCR法 ,从人睾丸组织中分离了人受精促进肽受体TCP11基因的一个新的剪切体TCP11b ,它编码 5 0 3个氨基酸的蛋白质 ,与TCP11a相比 ,在基因组的 5′端存在复杂的外显子剪接现象。运用荧光原位杂交 (FISH)方法 ,显示该基因定位到人染色体 6p2 1。Northern杂交及多组织RT PCR的结果显示该转录本在正常睾丸中表达 ,而其他组织、无精症患者及胎儿睾丸组织中未见该基因的表达。该结果结合mTcp 11功能的提示 ,TCP11b这种转录本对精子发生和人受精过程可能起重要作用。

Cloning, Expression and Alternative Splicing of a Novel Isoform of Human TCP11b Gene

Based on homology analysis and RT-PCR, a novel isoform of human TCP11 gene was isolated. It encodes a 503 amino acid protein that is highly homologous to the mouse 566 amino acid protein Tcp-11. Tcp-11 is important to sperm function because it may be the receptor of fertilization promoting peptide (FPP). Complicated alternative splicing was found to exist between TCP11a and TCP11b genes. The gene has been mapped to human chromosome band 6p21 by fluorescence in situ hybridization. Results of Northern blot and RT-PCR analysis of RNA extracted from human tissues revealed that the gene was expressed in fertile adult testes only, but neither in azoospermic patient testes, fetal testes nor in other human tissues. Our results suggest that TCP11b gene may be important to human sperm function and male fertility.