Nanoparticle-based electrochemical detection of hepatitis B virus using stripping chronopotentiometry |
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Authors: | Hanaee H Ghourchian H Ziaee A-A |
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Institution: | Institute of Biochemistry and Biophysics, University of Tehran, Tehran 1981813563, Iran. |
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Abstract: | A selective and sensitive gold nanoparticle-based electrochemical method for detection of hepatitis B virus DNA sequences was used. This method relies on the hybridization of amplified hepatitis B virus DNA strands with probes that are extended on paramagnetic beads. After separation of noncomplementary sequences, hybridized magnetic beads were treated with streptavidin-modified gold followed by silver enhancement. High selectivity and high sensitivity were obtained using electrochemical stripping detection of silver ions that were deposited on gold nanoparticles. With a signal/noise ratio of approximately 4.6, the detection limit was estimated to be 0.7ng/ml. |
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Keywords: | Gold nanoparticles Paramagnetic beads Silver enhancement Stripping chronopotentiometry Hepatitis B virus |
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