| 臭味假单胞菌邻苯二酚1,2—双加氧酶的制备和一般性质 |
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| 引用本文： | 冠秀芬,李钦.臭味假单胞菌邻苯二酚1,2—双加氧酶的制备和一般性质[J].微生物学报,1990,30(5):397-399. |
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| 作者姓名： | 冠秀芬 李钦 |
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| 摘 要： |
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| 关 键 词： | 臭味假单孢菌 加氧酶 |
The preparation and properties of catechol-1,2-dioxygenase from Pseudomonas putida |
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| X Kou,Q Li.The preparation and properties of catechol-1,2-dioxygenase from Pseudomonas putida[J].Acta Microbiologica Sinica,1990,30(5):397-399. |
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| Authors: | X Kou Q Li |
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| Institution: | Institute of Microbiology, Academia Sinica, Beijing. |
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| Abstract: | Catechol-1,2-dioxygenase (EC 1.13.11.1) catalyzes the degradation of catechol to cis, cis-muconic acid. The biochemical properties of catechol-1,2-dioxygenase from Pseudomonas putida 84103 were investigated. The optimum pH and temperature is 7.5-8.0 and 25-30 degrees C, respectively. Cu2+, Zn2+ inhibit the enzyme activity. The paper chromatograph and UV absorption spectrum of enzymatic reaction product are accordance with those of the standard muconic acid. |
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