类鼻疽菌血清分型

类鼻疽假单胞菌根据不耐热抗原的有无分为血清I型和II型。在没有标准血清情况下，用吸收试验，选出产不耐热抗原较好的菌株，用scphadex G—200纯化抗原制备I型血清，用该血清对我国分离的68株及引进6株菌以琼脂扩散法，进行血清学分型。结果表明：68株为血清I型，3株为血清II型菌，3株不稳定。上述结果与文献报道的一致。即血清I型菌多存在于亚洲，血清型与菌株来源(环境、动物)无关，但与地理分布有关。

Classify species of Pseudomonas pseudomallei into serotypes

Species of P. pseudomallei can be classified into two serotypes, serotype I and serotype II, based on whether or not it contains a thermolabile antigen beside a thermostable one. Under the condition of lack of typing serum, by means of serum absorption test, we recognized a strain which contains a major thermolabile antigen. The antigen was purified by Sephadex G-200, and it was used to inoculate rabbits. With the immunoserum at hand, we identified 68 of the domestic chinese strains and 6 of alien strains for serotyping by bi-directional agar diffusion test. The results showed that 68 strains were identified serotype I, 3 strains serotype II, and the remaining 3 comparable with those reported indicating that strains of serotype I were found mostly in Asia, and that the serotype are unrelated to their existing environments, nor that of animal bodies, but connected with their existence in geographic distribution.