Relative speed of fixation of glutaraldehyde and osmic acid in plant cells measured by grana appearance in chloroplasts |
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| Authors: | W G Langenberg |
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| Institution: | (1) Agricultural Research Service, U.S. Department of Agriculture, North Central Region, USA;(2) Present address: Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska, USA |
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| Abstract: | Summary Brief fixation in a mixture of glutaraldehyde and OsO4 caused stacked chloroplast grana membranes in leaf cells of wheat, barley, tobacco, maize, cowpea, pigweed or bean plants to distend and vesiculate. Fixation with glutaraldehyde followed by OsO4 prevented this fixation artifact. In a fixative mixture, OsO4 apparently reacted with cell contents before glutaraldehyde. |
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