Map-based cloning and characterization of a gene controlling hairiness and seed coat color traits in <Emphasis Type="Italic">Brassica rapa</Emphasis> |
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Authors: | Jiefu Zhang Ying Lu Yuxiang Yuan Xiaowei Zhang Jianfeng Geng Yu Chen Sylvie Cloutier Peter B E McVetty Genyi Li |
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Institution: | (1) Department of Plant Science, University of Manitoba, Winnipeg, Canada, R3T 2N2;(2) Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China;(3) Henan Academy of Agricultural Sciences, Zhengzhou, 450001, China;(4) Cereal Research Center, Agriculture Agri-Food Canada, Winnipeg, Canada, R3T 2N2 |
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Abstract: | A glabrous, yellow-seeded doubled haploid (DH) line and a hairy, black-seeded DH line in Chinese cabbage (B. rapa) were used as parents to develop a DH line population that segregated for both hairiness and seed coat color traits. The
data showed that both traits completely co-segregated each other, suggesting that one Mendelian locus controlled both hairiness
and seed coat color in this population. A fine genetic map was constructed and a SNP marker that was located inside a Brassica ortholog of TRANSPARENT TESTA GLABRA 1 (TTG1) in Arabidopsis showed complete linkage to both the hairiness and seed coat color gene, suggesting that the Brassica
TTG1 ortholog shared the same gene function as its Arabidopsis counterpart. Further sequence analysis of the alleles from hairless,
yellow-seeded and hairy, black-seeded DH lines in B. rapa showed that a 94-base deletion was found in the hairless, yellow-seeded DH lines. A nonfunctional truncated protein in the
hairless, yellow-seeded DH lines in B. rapa was suggested by the coding sequence of the TTG1 ortholog. Both of the TTG1 homologs from the black and yellow seeded B. rapa lines were used to transform an Arabidopsis ttg1 mutant and the results showed that the TTG1 homolog from the black seeded B. rapa recovered the Arabidopsis ttg1 mutant, while the yellow seeded homolog did not, suggesting that the deletion in the Brassica TTG1 homolog had led to the yellow seeded natural mutant. This was the first identified gene in Brassica species that simultaneously
controlled both hairiness and seed coat color traits. |
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Keywords: | Brassica rapa Seed coat color Hairiness Map-based cloning TTG1 homolog |
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