人肝脏组织亚细胞组分中含硒蛋白的分离与测定

硒是生物必需的微量元素 ,具有重要的生物化学功能 ,与人类和动物的健康及疾病密切相关1,2 ] .生物体内 80 %以上的硒是以与蛋白质结合的形式存在 ,所以目前大量的研究集中在硒蛋白的分离、鉴定和功能研究等方面3 ] .从 1998年起 ,我们对正常人肝组织中硒和含硒蛋白的亚细胞分布作了一些探索性的工作 ,发现含硒蛋白在各亚细胞组分中的分布有显著的不同4 ,5] .前期工作采用的凝胶过滤柱层析法研究含硒蛋白 ,该法分离量大 ,但分辨率较差 ,分子量相近的蛋白质难以区分4 ,5] .本工作采用ＳＤＳ 聚丙烯酰胺凝胶电泳 (ＳＤＳ ＰＡＧＥ)分离人…

Isolation and Determination of Selenium-containing Proteins in Subcellular Fractions of Human Liver

The distribution of selenium\|containing proteins in the subcellular fractions of human liver components was treated by sodium dodecyl sulfate\|polyacrylamide gel electrophoresis (SDS\|PAGE) and hydride generation\|atomic fluorescence spectrophotometry (HG\|AFS). About 24 kinds of Se\|containing proteins or protein subunits were found in subcellular fractions. Their molecular weights were mostly ranged from 20 to 30 kD and from 50 to 80 kD. Among them, about 61 kD and 21 kD Se\|containing proteins existed in each fraction with higher concentration. The 54 kD Se\|containing protein existed in nuclei, mitochondria, microsome and cytosol. The specific subcellular distributions of Se\|containing proteins suggest that Se could play specific important biological roles in each organelle.