Hyaluronic acid production in Bacillus subtilis |
| |
Authors: | Widner Bill Behr Régine Von Dollen Steve Tang Maria Heu Tia Sloma Alan Sternberg Dave Deangelis Paul L Weigel Paul H Brown Steve |
| |
Institution: | Novozymes, Inc., 1445 Drew Avenue, Davis, CA 95616, USA. wwi@novozymes.com |
| |
Abstract: | The hasA gene from Streptococcus equisimilis, which encodes the enzyme hyaluronan synthase, has been expressed in Bacillus subtilis, resulting in the production of hyaluronic acid (HA) in the 1-MDa range. Artificial operons were assembled and tested, all of which contain the hasA gene along with one or more genes encoding enzymes involved in the synthesis of the UDP-precursor sugars that are required for HA synthesis. It was determined that the production of UDP-glucuronic acid is limiting in B. subtilis and that overexpressing the hasA gene along with the endogenous tuaD gene is sufficient for high-level production of HA. In addition, the B. subtilis-derived material was shown to be secreted and of high quality, comparable to commercially available sources of HA. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|