Purification and cloning of a novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis |
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Authors: | Liu Zhigang Liu Huan Liu Xiaoyu Wu Xuli |
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Affiliation: | aCollege of Life Sciences, Shenzhen University, Shenzhen 518060, Guangdong, China;bKey Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China |
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Abstract: | A novel antimicrobial peptide named as ixosin-B was isolated from the salivary glands of the hard tick, Ixodes sinensis, by gel filtration, ion exchange chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined as QLKVDLWGTRSGIQPEQHSSGKSDVRRWRSRY by Edman degradation. The cDNA encoding ixosin-B was cloned by cDNA library screening. The predicted protein from the cDNA sequence composed of 89 amino acids including mature ixosin-B. Purified ixosin-B exerted its antimicrobial activities against bacteria and fungi. No similarity was found by BLAST search to any database entries and, thus, our findings describe a novel antimicrobial peptide. It is also the fourth family of antimicrobial peptide from hard ticks. |
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Keywords: | Ticks Antimicrobial peptide Ixodes sinensis Salivary gland |
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