Cloning and characterization of a plasmid DNA from anacystis nidulans 6301 |
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Authors: | K Shinozaki N Tomioka C Yamada M Sugiura |
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Affiliation: | National Institute of Genetics, Mishima, Shizuoka-ken 411 Japan |
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Abstract: | A plasmid DNA of Anacystis nidulans 6301 was isolated by CsCl-EtBr centrifugation. The Mr of the plasmid, named pBA1, was estimated to be 5.04 +/- 0.26 X 10(6) by electron microscopic analysis and 5.2 X 10(6) by agarose gel electrophoresis. The pBA1 DNA was opened at a unique site with BamHI and cloned in pBR322 vector propagated in Escherichia coli HB101 cells. The recombinant plasmid, named pBAS18, was digested with various restriction endonucleases and its cleavage map was constructed. Based on this result, the cleavage map of the pBA1 plasmid is presented. |
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Keywords: | Recombinant DNA cyanobacterial plasmid cleavage map ampicillin resistance EtBr ethidium bromide RF replicating form tetracycline sensitivity |
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