Composition and phylogenetic analysis of wheat cryptochrome gene family |
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| Authors: | Pei Xu Hui Lan Zhu Hai Bin Xu Zheng Zhi Zhang Cai Qin Zhang Li Xia Zhang Zheng Qiang Ma |
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| Institution: | (1) The Applied Plant Genomics Lab, Crop Genomics and Bioinformatics Center, Nanjing Agricultural University, 210095 Jiangsu, People’s Republic of China;(2) National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095 Jiangsu, People’s Republic of China;(3) College of Agricultural Sciences, Nanjing Agricultural University, 210095 Nanjing, Jiangsu, People’s Republic of China;(4) Present address: Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, People’s Republic of China; |
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| Abstract: | Cryptochrome (CRY) gene family encodes photoreceptors mediating developmental responses to blue light throughout the life
of plants. We report here the characterization of CRY gene family in hexaploid wheat. Degenerate PCR amplification of the regions encoding the conserved flavin-binding domain
of CRY proteins yielded seven bands, resulting from amplification of CRY1a, CRY1b and CRY2 homologous genes. Assignment of individual amplicons to subgenomes was accomplished by comparing their sequence compositions
with those from the ancestor species of wheat. ESTs coding for CRY-DASH like proteins were identified in wheat EST database
in GenBank. Southern blot showed that TaCRY1a, TaCRY1b and TaCRY2 are single copy genes. We mapped TaCRY1a and TaCRY2 to chromosomes of homoeologous group 6, TaCRY1b to group 2, and TaCRY-DASH to group 7. Phylogenetic analysis showed that CRY subfamily diversification occurred before the divergence of monocots and dicots. The regulatory and functional changes of
CRY members within subfamily are discussed. |
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