Hydrogen fluoride effects on plasma membrane composition,ATPase activity and cell structure in needles of eastern white pine (Pinus strobus) seedlings |
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Authors: | Krzysztof J. Rakowski Janusz J. Zwiazek Michael J. Sumner |
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Affiliation: | (1) Department of Genetics and Forest Trees Physiology, Forest Research Institute, Bitwy Warszawskiej 1920R-3, PL-00-973 Warsaw, Poland;(2) Department of Forest Science, University of Alberta, T6G 2H1 Edmonton, Alberta, Canada;(3) Department of Botany, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada |
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Abstract: | Eastern white pine (Pinus strobus L.) seedlings were grown in controlled environment growth cabinets and fumigated with 0.4 and 1.6 g m–3 hydrogen fluoride for 2–28 days. Plasma membranes were isolated from needles of treated and control seedlings and their chemical composition and ATPase activity examined to determine early effects of hydrogen fluoride action. In plants treated for 2 days with both fluoride levels, ratios of plasma membrane free sterols:phospholipids and sterols:proteins were drastically higher than ratios in control plants. Seedlings treated with hydrogen fluoride for 8 days contained plasma membranes with elevated phospholipid:protein and sterol:protein ratios and their plasma membrane ATPase activity was higher than that of control plants. Prolonged, 28-day hydrogen fluoride treatment with 1.6 g m–3 level was the only treatment which produced a drastic inhibition of plasma membrane ATPase activity. During the initial stages of hydrogen fluoride treatment, treated cells did not show alterations of ultrastructure which were previously shown in cells of plants treated with soil applied sodium fluoride. The results of the present study indicate that the plasma membranes may be among the initial sites of hydrogen fluoride injury to plants as well as initial sites of defense reaction. |
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Keywords: | Plasma membrane Fluoride ATPase Sterols |
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