Conditions for quantitative transformation inAcinetobacter calcoaceticus |
| |
Authors: | J A Cruze J T Singer W R Finnerty |
| |
Institution: | (1) Department of Microbiology, University of Georgia, 30602 Athens, Georgia, USA |
| |
Abstract: | A transformation procedure that yielded high efficiencies was developed forAcinetobacter calcoaceticus. Strain BD413 Ura−
trpE was transformed to tryptophan prototrophy using highly purified DNA. Experimental parameters studied were: (i) recipient
cell concentration, (ii) DNA concentration, (iii) growth phase of the recipient cell population, (iv) composition of the growth
and transforming medium, and (v) time of incubation of recipient cells with donor DNA. Strain BD413 was competent for transformation
throught the growth cycle, with highest competence occurring early in the exponential phase of growth. Maximal transformation
efficiencies of 0.5% to 0.7% were obtained in media supporting rapid growth. Recipient cell concentrations of 1×106 to 6×106 cells/ml yielded the highest transformation frequencies, regardless of DNA concentration. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|