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Randomly amplified polymorphic DNA (RAPD) profiles for the distinction of Lactobacillus species
Authors:Ayele Nigatu  Siv Ahrné  Göran Molin
Affiliation:(1) Ethiopian Health and Nutrition Research Institute, P.O. Box 80928, Addis Ababa, Ethiopia;(2) Laboratory of Food Hygiene, Department of Food Technology, Chemical Centre, Lund University, P.O.Box 124, S-221 00 Lund, Sweden
Abstract:Forty-one type and reference strains of Lactobacillus were evaluated using their randomly amplified polymorphic DNA band profiles. Developed bands for each strain were distinct and enabled discrimination. The best correlations were obtained applying the Pearson product moment correlation coefficient (r) together with the unweighted pair group method using arithmetic averages algorithm. All of the strains were clearly differentiated at and below the 72% similarity value. Species discrimination might be possible making use of the distinctly polymorphic bands amplified specific to a strain.
Keywords:identification  Lactobacillus  RAPD  taxonomy  type strains
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