| 籽瓜种子蛋白组分和生化特性的研究(简报) |
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| 引用本文： | 张玉秀,赵文明. 籽瓜种子蛋白组分和生化特性的研究(简报)[J]. 分子细胞生物学报, 1991, 0(3) |
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| 作者姓名： | 张玉秀 赵文明 |
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| 作者单位： | 西北农业大学植物分子生物学研究室，西北农业大学植物分子生物学研究室 陕西 712100，陕西 712100 |
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| 基金项目： | The project supported by National Natural Scielice Foundation of China |
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| 摘 要： | 籽瓜是西瓜的变种,其种子板大、味香,是人们喜爱的食品。籽瓜种子蛋白质含量约为38%,其中78%是球蛋白。Blagrove(1980)报道。南瓜、西瓜、甜瓜及黄瓜等瓜类种子球蛋
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| 关 键 词： | 籽瓜 球蛋白 同源性 |
STUDIES ON THE PROTEIN COMPONENTS AND BIOCHEMICAL CHARACTER FROM SEEDS OF SEEDY WATERMELON |
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| Zhang Yu-xiu Zhao Wen-ming. STUDIES ON THE PROTEIN COMPONENTS AND BIOCHEMICAL CHARACTER FROM SEEDS OF SEEDY WATERMELON[J]. Journal of Molecular Cell Biology, 1991, 0(3) |
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| Authors: | Zhang Yu-xiu Zhao Wen-ming |
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| Abstract: | The dry seeds of four varieties of seedy watermelon were used as experimental materials. The seed cucurbitin from seedy watermelon were analyzed by SDS-PAGE and IEF-SDS-PAGE. The results showed that the cucurbitin has a molecular mass of 55 kD, it can be dissociated into six subunits of MW. 37kD, 35kD, 33kD, 23kD, 19 kD, and 18 kD in SDS-PAGE under reducing conditions. The 37 to 33 kD proteins are acidic subunit and their isoelectric points lay between pH 5.5 and 6.2, and the 23 to 18 kD proteins are basic subunit and their isoelectric points lay between pH 8.9 and 9.6. The three acidic subunits and the three basic subunits linked by disulphide bonds were also found in the seed cucurbitin. Therefore it is proposed that cucurbitin of seedy watermelon are homologous to leguminlike protein. |
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| Keywords: | Seedy Watermelon. Cucurbitin. Homology. |
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