Expression and production of bioactive human interleukin-18 in transgenic tobacco plants |
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Authors: | Bin Zhang Ying-Hua Yang Yong-Ming Lin Qing Rao Guo-Guang Zheng Ke-Fu Wu |
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Institution: | (1) National Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, P.R. China |
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Abstract: | The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05% of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN- on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g–1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo. |
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Keywords: | bioactivity human IL-18 interleukin-18 tobacco transgenic plants |
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