Anisakis pegreffii: a quantitative fluorescence PCR assay for detection in situ |
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Authors: | Fang Wenzhen Liu Fan Zhang Shaolei Lin Junhua Xu Shisan Luo Damin |
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Institution: | aSchool of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China;bKey Laboratory for Subtropical Wetland Ecosystem Research, Xiamen University, Ministry of Education, Xiamen, Fujian 361005, China |
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Abstract: | To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single L3 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground. |
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Keywords: | Anisakis pegreffii Food-borne parasitic zoonosis In situ Nematode Second internal transcribed spacer (ITS-2) TaqMan real-time PCR |
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