|
|||||
|
|
Molecular cloning and characterization of a calreticulin cDNA from the pinewood nematode Bursaphelenchus xylophilus |
|
| Authors: | Li Xundong Zhuo Kan Luo Mei Sun Longhua Liao Jinling |
| Affiliation: | aLaboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China;bYunnan Academy of Agricultural Sciences, 650205, China;cZhongkai University of Agriculture and Engineering, Guangzhou 510225, China;dBiomaterials and Biocatalysts Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore |
| Abstract: | The cloning and characterization of a cDNA encoding a calreticulin from the pinewood nematode Bursaphelenchus xylophilus is described herein. The full-length cDNA (Bx-crt-1) contained a 1200 bp open reading frame that could be translated to a 399 amino acid polypeptide. The deduced protein contained highly conserved regions of a calreticulin gene and had 66.2–70.1% amino acid sequence identity to other calreticulin sequences from nematodes. RNAi, RT-PCR amplification, and southern blot suggest that Bx-crt-1 may be important for the development of B. xylophilus. |
| Keywords: | cDNA cloning Bursaphelenchus xylophilus Calreticulin RACE RNAi RT-PCR Southern blot |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |