Trypanosoma evansi: Activities of adenine nucleotide degradation enzymes in cerebral cortex of infected rats |
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Authors: | Oliveira Camila B Spanevello Rosélia Da Silva Aleksandro S Souza Viviane C G Pimentel Victor C Thomé Gustavo R Schetinger Maria Rosa Lopes Sonia T A Leal Daniela B R Monteiro Silvia G |
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Institution: | aDepartment of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil;bDepartment of Chemistry, Universidade Federal de Santa Maria, Brazil;cDepartment of Small Animals, Universidade Federal de Santa Maria, Brazil |
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Abstract: | This study aimed to evaluate the activities of the ectoenzymes NTPDase and 5′-nucleotidase in synaptosomes from cerebral cortex of rats experimentally infected with Trypanosoma evansi. The animals were divided in four groups (n = 10) according to the time and degree of parasitemia (groups A, B, C and D). The animals from group A were euthanized on day 3 (low parasitemia), group B on day 5 (high parasitemia) and group C on day 15 (low parasitemia). Group D consisted of healthy rats (not-infected, n = 15) and were divided in three periods (n = 5) in order to compare with the infected groups. After euthanasia, cerebral cortex was removed for the preparation of synaptosomes and enzymatic assays. Group A showed no changes in enzymatic activities compared with control. The hydrolysis of ATP, ADP and AMP by the enzymes NTPDase and 5′-nucleotidase were increased (P < 0.05) in group B (38%, 140% and 61%, respectively) when compared with control. In the group C it was observed a decreased (22%) hydrolysis of ATP when compared with control group. The activities of NTPDase and 5′-nucleotidase in synaptosomes alters the acute phase of the disease when the number of circulating parasites is high, thus the change observed is probably due to the parasitemia. |
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Keywords: | Trypanosomosis NTPDase 5&prime -Nucleotidase |
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