Calcium signal regulates temperature-dependent transformation of sporozoites in malaria parasite development |
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| Authors: | Doi Yuko Shinzawa Naoaki Fukumoto Shinya Okano Hideyuki Kanuka Hirotaka |
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| Affiliation: | aNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan;bDepartment of Physiology, School of Medicine, Keio University, Shinanomachi, Shinjuku, Tokyo 160-8582, Japan;cDepartment of Molecular Parasitology, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan |
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| Abstract: | The infection by the malaria parasite of its mammalian host is initiated by the asexual reproduction of the parasite within the host hepatocyte. Before the reproduction, the elongated sporozoites undergo a depolarizing morphogenesis to the spherical exo-erythrocytic form (EEF). This change can be induced in vitro by shifting the environmental conditions, in the absence of host hepatocytes. Using rodent malaria parasites expressing a FRET-based calcium sensor, YC3.60, we observed that the intracellular calcium increased at the center of the bulbous structure during sporozoite transformation. Modulators of intracellular calcium signaling (A23187 and W-7) accelerated the sporozoite-rounding process. These data suggest that calcium signaling regulates the morphological development of the malaria parasite sporozoite to the EEF, and support a fundamental role for calcium as a universal transducer of external stimuli in the parasitic life cycle. |
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| Keywords: | Plasmodium Calcium Morphology Sporozoite Malaria |
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