Detection, distribution and selection of microsatellites (SSRs) in the genome of the yeast Saccharomyces cerevisiae as molecular markers |
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| Authors: | M.A. Pé rez,F.J. Gallego,I. Martí nez,& P. Hidalgo |
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| Affiliation: | Instituto Madrile?o de Investigación Agraria y Alimentaria (IMIA.), Comunidad de Madrid, Finca El Encín, Apdo. 127, 28800 Alcalá de Henares, Madrid. Spain. |
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| Abstract: | AIMS: The aim of this work was the selection of six polymorphic microsatellite loci for their use as molecular markers in the identification, typification and genetic differentiation of S. cerevisiae strains. METHODS AND RESULTS: The selection was undertaken following a search of the genomic DNA database of Saccharomyces cerevisiae for simple tandem repeat sequences (microsatellites) of di- and trinucleotides. The genetic variability generated by these markers was evaluated in 51 isolates. The discriminatory power produced by combining the information obtained by the six microsatellites was very high. A total of 57 alleles, which generated 44 genotypes, were found. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: The multiple analysis of microsatellites proved to be a powerful and agile tool for analysing the genome of S. cerevisiae populations. |
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