| 野油菜黄单胞菌S-152内切葡聚糖酶基因在大肠杆菌中的克隆与表达 |
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| 引用本文： | 汪天虹,钟玲,王春卉,朱悦WANG Tian-hong,ZHONG Ling,WANG Chun-hui,ZHU Yue.野油菜黄单胞菌S-152内切葡聚糖酶基因在大肠杆菌中的克隆与表达[J].遗传,1998,20(5):20-24. |
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| 作者姓名： | 汪天虹 钟玲 王春卉 朱悦WANG Tian-hong ZHONG Ling WANG Chun-hui ZHU Yue |
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| 作者单位： | 1.山东大学微生物技术国家重点实验室,济南 250100
2.友谊医院病理室,北京 100050
1.National Key Microbiological Laboratory,Shandong University,Jinan 250100
2.Pathology Research Section,Friendship Hospital,Beijing 100050 |
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| 摘 要： | 以质粒pUC9为载体,大肠杆菌JM83为宿主菌,用鸟枪法克隆到纤维素降解细菌野油菜黄单胞菌S-152的内切葡聚糖酶(CMCase)基因,克隆到的CMCas e基因位于2.7kb的HindⅢ片段上,该重组质粒命名为pUC9H-1。Southern印迹杂交分析结果显示所克隆到的内切葡聚糖酶基因与野油菜黄单胞菌染色体DNA有亲和性。对克隆株的酶学性质分析表明,其CMCase活力为0.310μmol Glu/mg蛋白·分钟,最适作用pH为6.4,最适作用温度为55℃。
Abstract:Using plasmid pUC9 as vector,Escherichia coli JM83 as host strain,an endoglucanase(CMCase)gene has been cloned by Shoot-gun method from cellulose-degrading bacteria Xanthomonas campestris S-152,The recombinant plasmid pCU9H-1 was isolated from the positive transformant producing CMCase.The CMCase gene located in a 2.7kb HindIII DNA fragment.The result of Southern hybridization indicated that the CMCase gene cloned has affinity to chromosome DNA of Xanthomonas campestris S-152.The analysis of enzymatic activity of CMCase positive clone JM83(pUC9H-1)was carried out and the result indicated that the CMCase activity was about 0.310μmol Glu/mg pro·min,the optimum pH was 6.4 and the optimum temperature was 55℃.
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| 关 键 词： | 野油菜黄单胞菌 内切葡聚糖酶基因 基因克隆 Key words Xanthomonas campestris Endoglucanase gene 雄核发育 大鳞副泥鳅 热休克 二倍体克隆鱼 泥鳅 |
Cloning and Expression of Endoglucanase Gene of Xanthomonase campestris in Escherichia coli |
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| WANG Tianhong,ZHONG Ling,WANG Chunhui.Cloning and Expression of Endoglucanase Gene of Xanthomonase campestris in Escherichia coli[J].Hereditas,1998,20(5):20-24. |
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| Authors: | WANG Tianhong ZHONG Ling WANG Chunhui |
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| Abstract: | |
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| Keywords: | Xanthomonas campestris Endoglucanase gene Gene cloning |
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