Rabbit antibodies against two different extracellular domains of human thyrotropin receptor possess thyroid stimulating activities |
| |
Authors: | T Endo M Ohmori M Ikeda S Kotani T Onaya |
| |
Institution: | Third Department of Internal Medicine, University of Yamanashi Medical School, Japan. |
| |
Abstract: | We have produced rabbit antibodies against synthetic peptides corresponding to the mid-region (amino acid residues 172-202, C peptide) and to the unique segment near the transmembrane region (amino acid residues 341-370, P peptide) in the extracellular component of the human thyrotropin (TSH) receptor and evaluated their biological activities. Both anti-C peptide antibodies raised in two rabbits showed strong thyroid stimulating activities (TSAb) (4127% and 2548%). Anti-P peptide antibodies raised in two rabbits were also strongly positive for TSAb activities (359% and 3468%). However, none of these antibodies had TSH-binding inhibitor immunoglobulin (TBII) activities. These results suggest that the domains responsible for TSAb are likely to span the entire extracellular component of the TSH receptor. |
| |
Keywords: | |
|
|