Production and purification of Clostridium botulinum type C and D neurotoxin |
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Authors: | Frank Gesslera,Helge Bö hnela |
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Affiliation: | Institute for Crop and Animal Production in the Tropics, Georg August University, G?ttingen, Germany. fgessle@gwdg.de |
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Abstract: | Neurotoxins of Clostridium botulinum are needed in basic neurologic research, but as therapeutic agent for certain neuromuscular disorders like strabism as well. A method for the production and purification of botulinum neurotoxins C and D is reported using a two-step hollow-fiber cross flow filtration and a newly developed chromatographic purification procedure. Hollow-fiber filtration proved to be a rapid and safe concentration and pre-purification step, which can easily be scaled up. The chromatographic purification included hydrophobic interaction, anion exchange and size exclusion chromatography runs. Botulinum neurotoxins C and D could be recovered with an overall yield of 12.6% and 10.6%, respectively. A specific toxicity of 1.86 x 10(7) minimal lethal dose mg(-1) (type C) and 5.26 x 10(7) minimal lethal dose mg(-1) (type D) was determined in the mouse bioassay. |
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Keywords: | Clostridium botulinum Neurotoxin purification Botulinum neurotoxin type C Botulinum neurotoxin type D |
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