The role of the exocyclic amino groups of conserved purines in hammerhead ribozyme cleavage. |
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Authors: | G Slim M J Gait |
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Affiliation: | MRC Laboratory of Molecular Biology, Cambridge, UK. |
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Abstract: | A series of 2 stranded hammerhead ribozymes has been synthesized in which single conserved adenosine residues have been replaced by nebularine or single guanosine residues by inosine. Comparison of the rates of trans-cleavage for the modified structures suggests the presence of interstrand non-Watson-Crick hydrogen bonding interactions including a GA:AG double mismatch. The exocyclic amino group of one of the guanosine residues is essential for cleavage. |
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