Lipid biosynthesis in cultures of oilseed rape |
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Authors: | Randall J Weselake |
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Institution: | (1) Department of Chemistry and Biochemistry, University of Lethbridge, 4401 University Dr, T1K 3M4 Lethbridge, Alberta, Canada |
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Abstract: | Summary This review focuses on how microspore-derived (MD) embros and cell suspension cultures of oilseed rape have been used to advance
our understanding of the biochemistry and molecular biology of lipid biosynthesis in plants. Both types of cultures are easily
maintained and circumvent the difficulties associated with using developing seeds for investigations of lipid biosynthesis.
Developing MD embryos exhibit a similar storage lipid accumulation profile and fatty acid composition to developing seed.
The use of dihaploids derived from plantlets of MD embryos have accelerated breeding programs and have proven useful in the
detection of recessive mutations. MD embryos and MD cell suspension cultures have been particularly useful in investigating
the properties of key enzymes involved in triacylglycerol (TG) bioassembly. MD cell suspension cultures, however, offer the
advantage of being able to study lipid metabolism in the absence of cellular differentiation. TG accumulation can be induced
in MD cell suspension cultures by increasing the sucrose concentration of the growth medium thereby providing a useful system
to investigate gene expression and the proteomics of lipid biosynthesis. |
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Keywords: | triacylglycerol accumulation microspore-derived embryos cell suspension Brassica |
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