| 猪生长激素及其单克隆抗体的制备与纯化 |
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| 引用本文： | 余慕贞,方是民,王韵,何新,史瀛仙. 猪生长激素及其单克隆抗体的制备与纯化[J]. 中国生物化学与分子生物学报, 1992, 8(5): 587-592 |
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| 作者姓名： | 余慕贞 方是民 王韵 何新 史瀛仙 |
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| 作者单位： | 中国科学院发育生物学研究所，中国科学院发育生物学研究所，中国科学院发育生物学研究所，中国科学院发育生物学研究所，中国科学院发育生物学研究所 北京 100080，北京 100080，北京 100080，北京 100080，北京 100080 |
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| 摘 要： | 本文介绍一种简便快捷提纯猪生长激素的方法。通过杂交瘤技术,首次获得抗猪生长激素的单克隆抗体,并讨论和比较从腹水纯化单克隆抗体的各种方法。
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| 关 键 词： | 猪生长激素 单克隆抗体 单抗纯化方法 |
| 收稿时间： | 1992-10-20 |
Preparation and Purification of Porcine Growth Hormone and Its Monoclonal Antibodies |
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| Yu,Mu-zhen Fang,Shi-min Wang,Yun He,Xin Shih,Ying-Xian. Preparation and Purification of Porcine Growth Hormone and Its Monoclonal Antibodies[J]. Chinese Journal of Biochemistry and Molecular Biology, 1992, 8(5): 587-592 |
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| Authors: | Yu Mu-zhen Fang Shi-min Wang Yun He Xin Shih Ying-Xian |
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| Affiliation: | (Institute of Developmental Biology,Academia Sinica,Beijing 100080 |
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| Abstract: | The porcine growth hormone of high purity was extracted and purified with goodyield from pituitaries by simple, rapid method without high speed centri-fugation.This hormone was used to immunize Balb/c mices and the monoclonal antibodies against porcine growth hormone was obtained for the first time by the technique of hybridoma.Various methods to isolate and purify the monocional antibody from ascites fluid, such as ammonium sulfate precipitation and chromatography with hydroxylapatite, CM-affi-gel-blue, DEAE-cellulose, protein A agarose and antigen affinity immunology, were compared and discussed. |
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| Keywords: | Porcine growth hormone Monoclonal antibody Purification method of monoclonal antibody |
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