Substrate specificity of Stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid |
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Authors: | In-Sik Yu Soo-Jin Yeom Hye-Jung Kim Jung-Kul Lee Yong-Hwi Kim Deok-Kun Oh |
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Affiliation: | (1) Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South Korea;(2) Department of Chemical Engineering, Konkuk University, Seoul, 143-701, South Korea;(3) Department of Food Science and Technology, Sejong University, Seoul, 143-747, South Korea |
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Abstract: | An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regio-specificity for C16 and C18 cis-9 unsaturated fatty acids. These fatty acids are converted to their 10-hydroxy fatty acids without detectable byproducts. Among the cis-9-unsaturated fatty acids, S. nitritireducens showed the highest specificity for linoleic acid. The cells converted 20 mM linoleic acid to 13.5 mM 10-hydroxy-12(Z)-octadecenoic acid at 30°C and pH 7.5 with a yield of 67.5% (mol/mol). |
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Keywords: | 10-Hydroxy fatty acids Substrate specificity Stenotrophomonas nitritireducens cis-9-Unsaturated fatty acids |
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