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The effect of virus infection on morphology and protein components of pollen grains
Authors:K Petrzik  J Špak  J Nebesářová  J Fránova
Institution:1. Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Brani?ovská 31, 370 05, ?eské Budějovice, Czech Republic
Abstract:Morphology of pollen grains collected from healthy and virus infected plants ofChenopodium quinoa L.,Chenopodium album L. andNicotiana tabacum L. cv. Samsun was investigated using scanning electron microscopy (SEM). Pollen grains from tobacco plans infected with tobacco mosaic virus (TMV) were smaller, with rounded shape and conspicuous deformation of aperture unlike oval and smooth pollen grains from healthy plants. No morphological alterations were observed inC. quinoa andC. album plants infected with TMV and cucumber mosaic virus (CMV). Polyacrylamide gel electrophoresis of pollen proteins revealed substantial quantitative and qualitative differences in protein components of pollen grains collected from healthy and virus infected plants
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