Hardening by abscisic acid of tobacco plantlets grownin vitro |
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Authors: | J. PosplŠllová |
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Affiliation: | 1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce la, 160 00, Prague 6, Czech Republic
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Abstract: | Tobacco plantlets were grownin vitro on Murashige and Skoog’s medium with2 % of saccharose. Addition of 0.01 mM abscisic acid (ABA) into the medium decreased stomatal conductance of the adaxial epidermis and especially the abaxial epidermis without negative effects on growth parameters. As a result the rate of water loss from ABA-treated plantlets taken out of cultivation vessels was slower than that of control plantlets. This could help their acclimation after transplantation toex vitro conditions. |
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