| Abstract: | The concentration of PGF in rabbit Graafian follicles increases at ovulation but the cell type responsible for PGF secretion has not been identified. We have found that a pure population of granulosa cells isolated from pre-ovulatory follicles of estrous rabbits secrete prostaglandin F in tissue culture (total secretion, 446 ng/10 days; 0.09 pg/cell/day). LH/FSH did not influence the rate of PGF secretion, but there was a 50% inhibition after dibutyryl cAMP treatment, and complete inhibition by indomethacin. These results indicate that granulosa cells could secrete the prostaglandin which accumulates in the follicle at ovulation, and that PGF secretion may be modified by the addition of cAMP to the medium. |
