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Analysis of the genusZea (Poaceae) using polymorphic chloroplast simple sequence repeats |
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| Authors: | Jim Provan Pat Lawrence George Young Frank Wright Robert Bird GianPaolo Paglia Federica Cattonaro Michele Morgante Wayne Powell |
| Institution: | (1) Department of Cell and Molecular Genetics, Scottish Crop Research Institute, DD2 5DA Invergowrie, Dundee, Scotland, UK;(2) Biomathematics and Statistics Scotland, Scottish Crop Research Institute, DD2 5DA Invergowrie, Dundee, Scotland, UK;(3) IICD, 27607 Raleigh, NC, USA;(4) Dipartimento di Produzione Vegetale e Tecnologie Agrarie, Università di Udine, Via dell' Scienze, 1-33100 Udine, Italy |
| Abstract: | We have used polymorphic chloroplast simple sequence repeats (cpSSRs) to analyse levels of diversity and relationships within the genusZea. Between two and nine alleles were found at 15 polymorphic loci and combining the data from these loci gave 32 haplotypes in the 37 accessions studied. Genetic differentiation between the two sections within the genus was calculated using the ST statistic which showed that  70% of the total variation was found to exist between the sections. A phylogenetic analysis based on the 
2 distance metric showed a large split between the two sections and suggested multiple origins of modern cultivated maizeZea mays subsp.mays. The agreement of the phylogenetic tree with other molecular, morphological and karyological studies suggests that cpSSRs may have value in phylogenetic studies in plants. |
| Keywords: | Poaceae Zea chloroplast simple sequence repeats diversity |
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