Electro-stimulated transformation of E. coli cells pretreated by EDTA solution |
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| Authors: | E S Cymbalyuk L V Chernomordik N E Broude |
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| Affiliation: | A.N. Frumkin Institute of Electrochemistry, USSR Academy of Sciences, Moscow. |
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| Abstract: | The phenomenon of transformation of E. coli cells under electric treatment has been studied. The cells of strains MH 1, HB 101 and DH 1 after EDTA treatment in an isotonic medium were transformed with DNA pBR322 by applying a single exponential pulse (E = 10 kV/cm, T = 1.5 ms) to the suspension. The maximum transformation efficiency obtained was 4 X 10(6) colonies/micrograms DNA. The maximum transformation frequency was 0.4% at a DNA concentration of 15 micrograms/ml. |
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