Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi |
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Authors: | Tamagawa Yuuki Hirai Hirofumi Kawai Shingo Nishida Tomoaki |
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Institution: | Department of Forest Resources Science, Faculty of Agriculture, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan. |
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Abstract: | Endocrine-disrupting genistein was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. Genistein decreased by 93% after 4 days of treatment and the activities of ligninolytic enzymes, manganese peroxidase (MnP) and laccase, were detected during treatment, thus suggesting that the disappearance of genistein is related to ligninolytic enzymes produced extracellularly by white rot fungi. Therefore, genistein was treated with MnP, laccase, and the laccase-mediator system with 1-hydroxybenzotriazole (HBT) as a mediator. HPLC analysis demonstrated that genistein disappeared almost completely in the reaction mixture after 4 h of treatment with either MnP, laccase, or the laccase-HBT system. Using the yeast two-hybrid assay system, it was also confirmed that three enzymatic treatments completely removed the estrogenic activity of genistein after 4h. These results strongly suggest that ligninolytic enzymes are effective in removing the estrogenic activity of genistein. |
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Keywords: | Genistein Estrogenic activity Ligninolytic enzyme Genistein Ligninolytic Manganese peroxidase Laccase White rot fungi |
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