Fractionation of protein components of plasma membranes from the electric organ of Torpedo marmorata |
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Authors: | Lena Elfman Edith Heilbronn Peter L Jrgensen |
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Institution: | 1. Unit of Neurochemistry and Neurotoxicology, University of Stockholm, Enköpingsvägen 126, S-172 46 Sundbyberg Sweden;2. Institute of Physiology, Aarhus University, DK-8000 Aarhus C Denmark |
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Abstract: | A procedure has been developed for the separation of intrinsic proteins of plasma membranes from the electric organ of Torpedo marmorata. (Na+ + K+)-ATPase, nicotinic acetylcholine receptor and acetylcholinesterase remained active after solubilization with the nonionic detergent dodecyl octaethylene glycol monoether (C12E8). These components could be separated by ion exchange chromatography on DEAE-Sephadex A-25. Fractions enriched in ouabain-sensitive K+-phosphatase or (Na+ + K+)-ATPase activity showed two bands in sodium dodecyl sulphate polyacrylamide gel electrophoresis corresponding to the α- and β-subunits. The (Na+ + K+)-ATPase was shown to have immunological determinants in common with a 93 kDa polypeptide which copurified with the nicotinic acetylcholine receptor, also after solubilization in Triton X-100 and chromatography on Naja naja siamensis α-toxin-Sepharose columns. The data suggest that the α-subunit of (Na+ + K+)-ATPase associates with the acetylcholine receptor in the membranes of the electric organ. |
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Keywords: | Acetylcholine receptor (Na+ + K+)-ATPase Electric organ Membrane protein (T marmorata) |
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