Transfer of a benomyl resistance marker by heat-inactivated Trichoderma reesei protoplasts |
| |
| Authors: | V Meza P Moreno R P Tengerdy M Gutierrez-Correa |
| |
| Institution: | (1) Laboratorio de Micologia y Biotecnologia, Universidad Nacional Agraria La Molina, Apartado 456, 1 Lima, Peru;(2) Department of Microbiology, Colorado State University, 80523 Fort Collins, CO, USA |
| |
| Abstract: | Summary Protoplasts from a benomyl resistant Trichoderma reesei mutant were heat inactivated at 60°C for 8 min and fused with viable protoplasts from an osmosensitive, non-sporulating T. reesei strain. Fusants recovered on 50  g/ml benomyl containing potato dextrose agar plates grew and sporulated well. Cellulolytic enzyme activities produced in liquid culture by selected fusants were higher than those produced by parental strains. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
