Dose-dependent effects of malformin A1 on IAA-induced ethylene production in mung bean (Vigna radiate L.) hypocotyl segments |
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Authors: | Kim Soon Young Cho A-young Kim Kun Woo Oh Seung -Eun |
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Affiliation: | (1) Department of Biological Sciences, Konkuk University, 143-701 Seoul, Korea;(2) Department of Biological Sciences, Andong National University, 760-749 Gyeongbuk, Korea;(3) School of Bioresource Science, Andong National University, 760-749 Gyeongbuk, Korea |
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Abstract: | Purified malformin A1 (cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-lle), a cyclicpentapeptide toxin fromAspergillus niger, was applied to the hypocotyl segments of mung bean (Vigna radiata L.) seedlings to investigate its role in regulating ethylene biosynthesis. Production of ethylene was induced by treating the plants with 0.1 mM indole-3-acetic acid (1AA). When 0.1 μM malformin A1 was then applied, ethylene production increased and the activities of two key enzymes for its biosynthesis, 1-aminocyclopropane-1-carboxylic acid (ACC)-synthase (ACS) and ACC-oxidase (ACO), were also stimulated. However, at levels of 1 or 10 μM malformin A1, both ethylene production and enzymatic activities were significantly reduced. In the case of ACO,in vitro activity was regulated by malformin A1, independent of ACS activity or the influence of IAA. Furthermore, the conjugate form of ACC, N-malonyl ACC, was significantly promoted by treatment with 0.1 μM malformin A1. These data suggest that malformin A1 can modulate ethylene production through diverse paths and that its effect depends on the concentration of the treatment administered. |
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Keywords: | 1-aminocyclopropane-1-carboxylic acid ACC-oxidase ACC-synthase lAA-induced ethylene production N-malonyl ACC malformin A1 |
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